On-line courses, or invited lectures



 

Part 1: Overview of technological platforms

• Targeted (panel)-sequencing

• Whole-exome sequencing

• Whole-genome sequencing

• Single-cell sequencing: Droplet-based

• Single-cell sequencing: Plate-based

Part 2: Computational framework

• Data processing workflow

• Quality control reporting

Part 3: Big data to biological insights

• Functional annotation of genetic variants

• Delineating germline and somatic variants

• Mutational signatures

• Variant tabulation for publication

Part 4: Case study

• Characterising inherited germline variants in cancer patients

• Characterising age-related somatic variants in the general population

Part 1: Overview of technologocal platforms

• Short-read RNA-sequencing

• Long-read RNA-sequencing

• Single-cell sequencing: Droplet-based

• Single-cell sequencing: Plate-based

• Microarray

Part 2: Computational framework

• Data processing workflow

• Quality control reporting

• Expression quantification

Part 3: Big data to biological insights

• Unsupervised clustering

• Differential gene expression analysis

• Differential isoform expression analysis

• Differential alternative splicing analysis

• Pathway enrichment analysis

Part 4: Case study

• Transcriptomic dysregulation in the haematopoietic stem cell compartmnet of juvenile myeloid monocytic leukaemia patients

Part 1: Overview of technological platforms

• ChIP-sequencing

• ATAC-sequencing

• Single-cell ATAC-sequencing

Part 2: Computational framework

• Data processing workflow

• Quality control reporting

• Peak detection and quantification

Part 3: Big data to biological insights

• Unsupervised clustering

• Differential peak analysis

• Pathway enrichment analysis

• Transcription factor motif discovery

Part 4: Case study

• Characterising DNA methylation landscape in Vwf-deficient mouse models

• Characterising chromatin conformation landscape in normal and malignant haematopoietic cells

Part 1: Overview of technological platforms

• Genome-wide SNP array for common variant discovery

• Whole-exome sequencing for rare variant discovery

• qPCR for target variant

Part 2: Compurational framework

• Data processing workflow

• Quality control reporting

• Genomic inflation factor

Part 3: Big data to biologiical insignts

• GWAS: Single variant common germline association analysis

• ExWAS: Single variant rare germline association analysis

• Gene-level association analysis

• Polygenenic risk scoring

• Mendelian randomisation

• Meta-analysis

• Survival analysis

Part 4: Case study

• Genetic association analysis of clonal haematopoiesis in ancestry diverse populations

• Whole-genome sequencing
• Targeted sequencing of coding region
• Genotyping of specific domains

• de novo assembly
• Consensus sequence generation
• Quality control reporting

• Phylogenetic analysis
• Nucleotide polymorphism analysis
• Amino acid polymorphism analyis

• Infectious disease surveillance of EV-D68 in Malaysia and beyond